{"@context": "https://schema.org", "@type": "Dataset", "@id": "https://doi.org/10.5878/17te-jg54", "identifier": [{"@type": "PropertyValue", "unitCode": "DOI", "value": "https://doi.org/10.5878/17te-jg54"}, {"@type": "PropertyValue", "unitCode": "https://www.rikstermbanken.se/termposter/1848/diarienummer", "unitText": "principalRegistrationNumber", "value": "SLU.genfys.2023.4.4.I\u00c4-2"}, {"@type": "PropertyValue", "unitCode": "DOI", "value": "https://doi.org/10.5878/8sgv-nn24"}], "version": "2", "name": [{"@value": "Dataset of confocal microscopy from plant samples - High-throughput characterization of cortical microtubule arrays response to anisotropic tensile stress", "@language": "en"}, {"@value": "Dataupps\u00e4ttning fr\u00e5n konfokalmikroskopi av v\u00e4xtprover \u2013 \u201dHigh throughput\u201d-karakterisering av kortikala mikrotubuli-n\u00e4tverks svar p\u00e5 mekanisk stress.", "@language": "sv"}], "description": [{"@value": "The data set contains Tiff Z-stacks from light-grown hypocotyls and cotyledons of cortical microtubule (CMT) reporter lines either with few or no ablated cells from a time series experiment. This data set was analyzed with a new semi-automated image analysis workflow we have developed to quantify CMTs reorganization in individual cells following an ablation (https://github.com/VergerLab/MT_Angle2Ablation_Workflow).\n\nThe dataset in the zip file was analyzed using the scripts on GitHub (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). A step by step describes and explains all the scripts of the image analysis procedure. The intermediate data generated by the analysis method can be found on zenodo (https://doi.org/10.5281/zenodo.7436075). \n\nThe documentation file Example_2D_Image.tif gives a visual representation from a typical z-stack.", "@language": "en"}, {"@value": "Datam\u00e4ngden inneh\u00e5ller Tiff Z-stackar fr\u00e5n transgena ljusodlade hypokotyler och hj\u00e4rtblad fr\u00e5n Arabidopsis thaliana uttryckande kortikala mikrotubuli (CMT)-reporterlinjer antingen med f\u00e5 eller inga d\u00f6dade celler fr\u00e5n ett tidsserieexperiment. Denna dataupps\u00e4ttning analyserades med ett nytt halvautomatiserat arbetsfl\u00f6de f\u00f6r bildanalys som vi har utvecklat f\u00f6r att kvantifiera CMT-omorganisation i enskilda celler efter en ablation. (https://github.com/VergerLab/MT_Angle2Ablation_Workflow).\n\nDatam\u00e4ngden i zip-filen analyserades med hj\u00e4lp av skripten p\u00e5 GitHub (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). Ett steg f\u00f6r steg beskriver och f\u00f6rklarar alla skript f\u00f6r bildanalysproceduren. De mellanliggande data som genereras av analysmetoden kan hittas p\u00e5 zenodo (https://doi.org/10.5281/zenodo.7436075).\n\nDokumentationsfilen Example_2D_Image.tif ger en visuell representation fr\u00e5n en typisk z-stack.", "@language": "sv"}], "creator": [{"@type": "Person", "@id": "https://orcid.org/0000-0002-8386-7763", "sameAs": "https://orcid.org/0000-0002-8386-7763", "name": "Elsa Demes", "email": "elsa.demes@slu.se", "affiliation": [{"@type": "Organization", "name": [{"@value": "Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology", "@language": "en"}, {"@value": "Sveriges lantbruksuniversitet, Institutionen f\u00f6r skoglig genetik och v\u00e4xtfysiologi", "@language": "sv"}]}]}, {"@type": "Person", "@id": "https://orcid.org/0000-0003-3643-3978", "sameAs": "https://orcid.org/0000-0003-3643-3978", "name": "St\u00e9phane Verger", "email": "Stephane.verger@slu.se", "affiliation": [{"@type": "Organization", "name": [{"@value": "Swedish 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