For hypocotyls:

Growth conditions in vitro:

. 48hours at 4°C in the dark -> 4 days under 16hr/8hr (light/dark) light condition
. growth media: 1MS; 1% sucrose; 0.5g/l MES pH5.7; 0.8% agar

Genotypes:

. GFP-MBD (Marc et al., 1998)
. mCit-MBD (Armezzani et al., 2018)
. GFP-TUA6 (Ueda et al., 1999)
. bot1-7 / GFP-MBD (Uyttewaal et al., 2012)

Imaging:

. Microscope: Zeiss LSM800 upright; Zen blue software 
. Objective: W Plan-Apochromat 40x/1.0 NA; water dipping
. Z-stacks: 0.5um Z steps
. Frame size: 1024x512 px (pixel size: 0.31um)
. Time series: 20min time interval

For cotyledons/pavement cells:

Growth conditions in vitro:

. 48hours at 4°C in the dark -> 3 days in the dark at room temperature -> 24 hours under 16hr/8hr (light/dark) light condition
. growth media: 1MS; 1% sucrose; 0.5g/l MES pH5.7; 0.8% agar

Genotype:

. GFP-MBD (Marc et al., 1998)

Imaging:

. Microscope: Zeiss LSM800 upright; Zen blue software 
. Objective: W Plan-Apochromat 40x/1.0 NA; water dipping
. Z-stacks: 0.5um Z steps
. Frame size: 1024x512 px (pixel size: 0.31um)
. Time series: 20min time interval