This readme file was generated on 2024-05-31 by Erika Sundell and provides a list and description of each file included in the dataset. ----------------------------------------- DATASET GENERAL INFORMATION ----------------------------------------- Title of dataset: Data from various stunning attempts for Nile tilapia (Oreochromis Niloticus) Creator(s)/Primary investigator(s): Erika Sundell, ORCID 0000-0002-6552-9373; Jeroen Brijs, ORCID 0000-0002-3671-5038; Albin Gräns, ORCID 0000-0003-0268-1199. Persistent identifier of dataset: https://doi.org/10.5878/59q2-7h64 Related publication for description of project, methods etc: ----------------------------------------- FILE OVERVIEW ----------------------------------------- "Repository data set for manuscript 'The quest for a humane protocol for stunning and killing Nile tilapia (Oreochromis niloticus)'.xlsx" Brief description of content: The file contains data that supports the manuscript 'The quest for a humane protocol for stunning and killing Nile tilapia (Oreochromis niloticus)'. The data revolves around stunning efectiveness for Nile tilapia upon stunning attempts using various stunning methods. Relationship between worksheets: The file is divided into four worksheets where each worksheet contain the data for one of the four tested stunning methods. Relationship to other data not included in this dataset: none ----------------------------------------- DATA-SPECIFIC INFORMATION FOR "Repository data set for manuscript 'The quest for a humane protocol for stunning and killing Nile tilapia (Oreochromis niloticus)'.xlsx" ----------------------------------------- License: Creative Commons Public Domain Dedication CC BY WORKSHEET 1: Bolt gun stunning (percussive stunning) Number of columns/variables: 9 Number of rows/cases: 6 Explanation of missing/empty data: NA Abbreviations: VERs = visually evoked responses Color coding: Green = PRE stun; Blue = POST stun; Yellow = light phase; Grey = dark phase; Pink = presence of VERs; Apricot = absence of VER Variable list: Fish: Fish ID for the percussive stunning method Amplitude light phase before stunning (mV): the amplitude of the average beta wave frequency of the EEG trace prior to stunning; the average is based on 37-60 s of measurements, format: mV. Amplitude light phase after stunning (mV): the amplitude of the average beta wave frequency of the EEG trace after stunning; the average is based on 60-80 s of measurements, format: mV. PRE stun light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) prior to stunning, format: mV. PRE stun dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) prior to stunning, format: mV. POST stun light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) post stunning, format: mV. POST stun dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) post stunning, format: mV. Amplitude quoate PRE stun: The quoate between the light and dark phase prior to stunning (e.g., PRE stun light/PRE stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. Amplitude quoate POST stun:The quoate between the light and dark phase after stunning (e.g., POST stun light/POST stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. WORKSHEET 2: Live chilling as a stunning method Number of columns/variables: 10 Number of rows/cases: 12 Explanation of missing/empty data: NA Abbreviations: VERs = visually evoked responses Color coding: Green = PRE stun; Blue = During live chilling; RED = During warming; Yellow = light phase; Grey = dark phase; Pink = presence of VERs; Apricot = absence of VER Variable list: Fish: Fish ID for the live chilling method Loss of VERs with chilling, VERs lost by live chilling?: denotes whether the fish lost VERs or not, format: yes/no. Loss of VERs with chilling, time to loss of VERs (s): the time it took VERs to be lost, format: s Recovery of VERs with warming, VERs recovered with warming?: denotes whether the fish recovered VERs or not, format: yes/no. Recovery of VERs with warming, time to recovery of VERs (min): the time it took VERs to be recovered, format: min Recovery of VERs with warming, time to recovery of VERs (s): the time it took VERs to be recovered, format: s Amplitude light phase before live chilling (mV): the amplitude of the average beta wave frequency of the EEG trace prior to chilling; the average is based on 60-80 s of measurements, format: mV. Amplitude light phase when VERs were lost during live chilling (mV): the amplitude of the average beta wave frequency of the EEG trace when VERs were lost during chilling; the average is based on 40-70 s of measurements, format: mV. Amplitude light phase when VERs return during warming (mV): the amplitude of the average beta wave frequency of the EEG trace when VERs are recovered during warming; the average is based on 20-60 s of measurements, format: mV. PRE stun light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) prior to chilling, format: mV. PRE stun dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) prior to chilling, format: mV. During live chilling light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) during chilling, format: mV. During live chilling dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) during chilling, format: mV. During warming light: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) during warming, format: mV. During warming dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) during warming, format: mV. Amplitude quoate PRE stun: The quoate between the light and dark phase prior to stunning (i.e., PRE stun light/PRE stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. Amplitude quoate live chilling: The quoate between the light and dark phase when VERs were lost during chilling (i.e., During live chilling light/During live chilling dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. Amplitude quoate during warming:The quoate between the light and dark phase when VERs recovered during warming (i.e., during warming light/during warming dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. WORKSHEET 3: Electrical stunning Number of columns/variables: 13 Number of rows/cases: 28 Explanation of missing/empty data: empty space Abbreviations: VERs = visually evoked responses Color coding: Green = PRE stun; Blue = POST stun; Yellow = light phase; Grey = dark phase; Pink = presence of VERs; Apricot = absence of VER Variable list: Fish: Fish ID for the electrical stunning method Amplitude EEG trace PRE stunning (mV): the amplitude of the average beta wave frequency of the EEG trace prior to stunning, format: mV Amplitude EEG trace during insult (mV): the amplitude of the average beta wave frequency of the EEG trace during the insult, format: mV Amplitude EEG trace POST stunning (mV): the amplitude of the average beta wave frequency of the EEG trace when the insult is deemed over after stunning, format: mV Voltage (V): The voltage applied into the water bath, format: V Current (A): The current applied into the water bath, format: A Electrid field (V cm-1): The electric field is caluclated based on the applied voltage and the separation between the electrodes, which was 11 cm (i.e., Voltage/11), format: V cm-1 Current density (A dm-2): The current density is calculated basde on the applied current and area of the electrodes (i.e., Current/electrode area, where the electrodes where 47 x 15 cm), format: A dm-1 Insult?: Denotes whether an insult was observed or not, format: yes/no Insult duration (s): the time the insult lasted, format: s VERs lost?: Denotes whether VERs were lost or not, format: yes/no VERs lost duration (s): the time that VERs were lost upon stunning (s), format: s Ventilation lost?: Denotes whether ventilation was lost or not, format: yes/no Ventilation regained?: the time it took for ventilation to start after stunning, format: min, or YES, NO, unclear if minutes are applicable. PRE stun light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) prior to stunning, format: mV. PRE stun dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) prior to stunning, format: mV. POST stun light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) post stunning, format: mV. POST stun dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) post stunning, format: mV. Amplitude quoate PRE stun: The quoate between the light and dark phase prior to stunning (e.g., PRE stun light/PRE stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. Amplitude quoate POST stun:The quoate between the light and dark phase after stunning (e.g., POST stun light/POST stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. WORKSHEET 4: Sequential approach Number of columns/variables: 12 Number of rows/cases: 16 Explanation of missing/empty data: NA Abbreviations: VERs = visually evoked responses; NA = not applicable Color coding: Green = PRE stun; Blue = POST stun; RED = when VERs return post stun; Yellow = light phase; Grey = dark phase; Pink = presence of VERs; Apricot = absence of VER Variable list: Fish: Fish ID for the electrical stunning method Protocol: The different electrical exposures delivered to the fish prior to throat cutting and placement in ice slurry, format: V Voltage (V): The voltage applied into the water bath, format: V Current (A): The current applied into the water bath, format: A Electrid field (V cm-1): The electric field is caluclated based on the applied voltage and the separation between the electrodes, which was 11 cm (i.e., Voltage/11), format: V cm-1 Current density (A dm-2): The current density is calculated basde on the applied current and area of the electrodes (i.e., Current/electrode area, where the electrodes where 47 x 15 cm), format: A dm-1 VERs lost?: Denotes whether VERs were lost or not, format: yes/no VERs regained?: denotes whether VERs were regained and if so, the time (s) to recovery of VERs, format: yes/ no and if yes, time in s Amplitude light phase before stunning (mV): the amplitude of the average beta wave frequency of the EEG trace prior to electrical exposure, format: mV. Amplitude light phase during throat cut (mV): the amplitude of the average beta wave frequency of the EEG trace during the throat cutting event post electrical exposure, format: mV. Amplitude light phase in ice slurry (mV): the amplitude of the average beta wave frequency of the EEG trace after fish is placed in ice slurry following electrical exposure and throat cutting, format: mV. Amplitude light phase when VERs return (mV): the amplitude of the average beta wave frequency of the EEG trace when VERs recover, format: (mV) or NA if VERs did not return PRE stun light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) prior to stunning, format: mV. PRE stun dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) prior to stunning, format: mV. POST stun light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) post stunning, format: mV. POST stun dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) post stunning, format: mV. When VERs return light: amplitude of the average beta wave frequency of the EEG trace during the light phase (light phase constituted the 200 ms of recordings that followed the light flash) when VERs have recovered, format: mV. When VERs reutrn dark: amplitude of the average beta wave frequency of the EEG trace during the dark phase (dark phase constituted the 200 ms of recordings that followed the light phase) when VERs have recovered, format: mV. Amplitude quoate PRE stun: The quoate between the light and dark phase prior to stunning (e.g., PRE stun light/PRE stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. Amplitude quoate POST stun when VERs are lost: The quoate between the light and dark phase after stunning (e.g., POST stun light/POST stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate. Amplitude quoate POST stun when VERs return:The quoate between the light and dark phase after stunning (e.g., POST stun light/POST stun dark), presence of VER is underlined, absence of VER is not underlined, format: quoate.