This seedling_performance_explanations.txt file was generated on 2021-04-13 by BODIL HÄGGSTRÖM and updated on 2021-04-20 by DCU and 2021-04-27 by SND GENERAL INFORMATION 1. Title of Dataset: Data from field inventory 2019 of survival and growth of Scots pine (Pinus sylvestris) seedlings in Northern Sweden 2. Author Information A. Principal Investigator Contact Information Name: Bodil Häggström Institution: Swedish University of Agricultural Sciences Address: Umeå Plant Science Centre Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences 90183 Umeå Sweden Email: bodil.haggstrom@slu.se B. Associate or Co-investigator Contact Information Name: Annika Nordin Institution: Swedish University of Agricultural Sciences Address: Umeå Plant Science Centre Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences 90183 Umeå Sweden Email: Annika.Nordin@slu.se 3. Date of data collection: 2019-08-07 to 2019-09-04 4. Geographic location of data collection: Site A: 61.058188 N 16.156471 E Site B: 62.067031 N 15.093088 E Site C: 63.945034 N 18.446800 E Site D: 63,952229 N 18,437388 E Site E: 64,182400 N 19,912070 E Site F: 64,264957 N 19,607098 E Site G: 64,309788 N 19,644946 E Site H: 65,616476 N 20,628659 E Site I: 66,375145 N 21,818558 E Site J: 66,644275 N 20,304017 E Site K: 67,088861 N 22,298432 E 5. Information about funding sources that supported the collection of the data: Funding of the field study data collection was provided by the Trees and Crops for Future program at the Swedish University of Agricultural Sciences SLU and the Knut and Alice Wallenberg Foundation. SHARING/ACCESS INFORMATION 1. Links to publications that cite or use the data: Not yet published 2. Was data derived from another source? Yes A. If yes, list source(s): Precipitation data: SMHI (2019) https://www.smhi.se/data/meteorologi/ladda-ner-meteorologiska-observationer/#param=precipitation24HourSum,stations=all accessed 2019-10-07 Names and coordinates for weather stations from where precipitation data is sourced for each site: Weather station data for site A: Åmot A 60.9614 N, 16.4279 E Weather station data for site B: Ytterhogdal 62.1817 N, 14.9478 E Weather station data for site C and D: Fredrika A 64.0744 N, 18.3619 E Weather station data for site E, F and G: Vindeln-Sunnansjönäs 64.1386 N, 19.7618 E Weather station data for site H: Älvsbyn A 65.6691 N, 21.0620 E Weather station data for site I: Talljärv D 66.2498 N, 22.3044 E Weather station data for site J: Murjek D 66.4771 N, 20.9263 E Weather station data for site K: Tarendo D 67.1661 N, 22.6287 E Length of growing seasons derived from SMHI vegetation period length map: SMHI (2020) https://www.smhi.se/kunskapsbanken/klimat/fenologi/vegetationsperiod-1.6270 accessed 2020-12-07, CC-BY 4.0 6. Recommended citation for this dataset: Bodil Häggström. Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology (2021). Data from field inventory 2019 of survival and growth of Scots pine (Pinus sylvestris) seedlings in Northern Sweden. Svensk nationell datatjänst. Version 1. https://doi.org/10.5878/4gf7-ay12 DATA & FILE OVERVIEW 1. File List: a. effects_position_APtreatment_data_leader_length.csv Content: Leader shoot measurements of Pinus sylvestris seedlings from sites A,B,C,D,F,G,H,I,J and K (site E excluded due to un-sufficient amount of measurements). For each site is specified the coordinates, altitude, planting date, date of inventory, soil type (= all sites are underlain by till but with variation in dominant particle fraction), moistclass, Site Index, Length of growing season, original pot size of the seedlings, treatment (fertilized = one dose arginine-phosphate added), planting position and length of leader shoot (cm). b. effects_position_APtreatment_data_survival.csv Content: Survival data of Pinus sylvestris seedlings from sites A-K (all sites). For each site is specified the coordinates, altitude, planting date, date of inventory, soil type (= all sites are underlain by till but with variation in dominant particle fraction), moistclass, Site Index, Length of growing season, amount of precipitation first 30 days after planting (mm), SMHI weather station from which the precipitation data was derived, original pot size of the seedlings, treatment (fertilized = one dose arginine-phosphate added), planting position, seedling alive 2019, seedlings dead 2019, survival in %, mean leader shoot length and number of measured seedlings for each site/treatment/planting position combination. 2. Relationship between files, if important: File a. contain measurements of approximately 50% of the seedlings registered as “Alive” in file b. 3. Additional related data collected that was not included in the current data package: The seedlings included in file a. were measured for height and stem-base diameter as well. These data were not used since these measurements was considered heavily influenced by original size. There were originally a few measurements for non-scarified from site A and from site E that was excluded from analysis because of very low number of measurements due to low survival at site A and a high number of damaged seedlings at site E. Inventories from three additional sites were originally made, but the choice of method of analyses made these data non-usable (at two of these sites the requirement was not fulfilled that both positions in prepared soil would be represented at all sites and the third site these two positions was very had to distinguish from each other, which made the data un-reliable and the decision was therefore made to exclude these data). 4. Are there multiple versions of the dataset? No. METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: Field inventory was performed in the end of the second growing season during August-September 2019. All seedlings that had any green needles were classified as living, seedlings with no green needles and missing seedlings were classified as dead. Leader shoot length was measured from the top branches to the top of the terminal bud. Every second alive and undamaged seedling was measured with random start at first or second seedling for each row. At sites where many seedlings were damaged or dead in any of the planting position-and treatment combinations, all remaining undamaged seedlings was measured from that combination. 2. Methods for processing the data: Survival data from sites that fulfilled the requirements of at least both planting positions derived from soil preparation (mineral soil and tilt) was represented was filtered out from the collected data. This was made in excel. Measurement data from sites where a minimum of 3 measurements was made for each planting position- and treatment combination was filtered out from the collected data. This was made in excel. 3. Instrument- or software-specific information needed to interpret the data: R-studio (version 1.3.1093) software were used in all statistical analyses (R Core Team, 2019). Analysis of variance (ANOVA) was performed for survival and growth using the R car-package. Following R-packages were used for creating graphs for visual interpretation of the data: Rmisc, ggplot2, ggpubr, dplyr, ggthemes and ggpmisc. 4. Environmental/experimental conditions: The data was retrieved from seedlings that had been planted at multiple field sites which include various environments. The environmental variables used in analyses are length of growing season, amount of precipitation and site index which are all specified for each site in the data files. Other environment variables that are specified for each site in the data files that were not used in analyses is soil type which varied between silty- sandy- or coarse till and moist-class which was dry (10 sites) or mesic (1 site). The inventory of survival and growth was performed following the second growing season in field which means that the seedlings have been exposed to varying environment conditions through two growing seasons and one winter. 5. Describe any quality-assurance procedures performed on the data: When less than 3 measurements (0-2) were retrieved from any planting position and treatment-combinations at any site, these data were excluded since this was considered too few to make reliable analyses from. This applies to measurements of seedlings in non-scarified at site A and all planting positions at site E. 6. People involved with sample collection, processing, analysis and/or submission: Bodil Häggström, Annika Nordin, Matej Domevscik and Jonas Öhlund. DATA-SPECIFIC INFORMATION FOR: [effects_position_APtreatment_data_leader_length.csv] 1. Number of variables: 16 2. Number of cases/rows: 1133 3. Variable List: Site: Sites A-K. Each location is given a letter from south to north. Latitude_N: Latitudinal coordinate WGS84 Longitude_E: Longitudinal coordinate WGS84 Altitude_mao: Altitude, meter above ocean Planting_date: Date when seedlings where planted for each site Inventory_2019: Date when survival and growth inventory was performed for each site Soiltype_till: All sites are located on till. Coarse, sandy or silty defines dominant particle fraction based on personal assessment Moistclass: dry (10 sites) or mesic (1 site) Site_Index: T16-T24, “T” stands for “Tall” = Swedish for “Pine”. Site index represents the productivity of the sites and is the estimated height of dominant trees at 100 years based on the productivity of the former stand, unit meter. Growingseason_days_SMHI: Length of growing season is the normal value based on 1961-1990 data, where the start of growing season is defined by the first day of the year when the diurnal mean temperature has been above 5 °C for four consecutive days and end is the last day of the last four days period when the diurnal mean temperature has been above 5 °C (SMHI, 2020). Precipitation_30_first_days_mm_SMHI: The amount of precipitation from date of planting and the following 30 days, unit mm. Data is retrieved from the closest available SMHI weather stations as listed in access/sharing information, which means some clustered sites share the same data. SMHI_station: Name and coordinates for the weather station from where precipitation data was retrieved. Potsize_cm3: The size of the pot that seedlings were grown in, defining the substrate size in which all roots are confined at planting, unit cubic cm (cm3). Treatment: Fertilized = one dose of granular arginine-phosphate (arGrow® Granulat, Arevo AB, Umeå, Sweden) was added in the bottom of the planting hole together with each plant at the time of planting, b) untreated: no nutrients added. One dose arGrow® Granulat contains 40 mg N and 22 mg P, the active substance is L-arginine phosphate (C6H17N4O6P). Untreated: no fertilizer added. Position: Tilt= turned over humus tilt with mineral soil cover on top of intact humus. Mineral= bared mineral soil adjacent to the turned over tilt where topsoil was removed. Non_prepared= intact humus where no topsoil or vegetation was removed. Positions “Tilt” and “Mineral” both resulted from mechanical soil preparation, performed in 2017 either by mounding or disc-trenching. Leader_2019_cm: Leader shoot length of seedlings measured from top branches to top of terminal bud, unit cm. 4. Missing data codes: None. DATA-SPECIFIC INFORMATION FOR: [effects_position_APtreatment_data_survival.csv] 1. Number of variables: 20 2. Number of cases/rows: 64 3. Variable List: Site: Sites A-K. Each location is given a letter from south to north. Latitude_N: Latitudinal coordinate WGS84 Longitude_E: Longitudinal coordinate WGS84 Altitude_mao: Altitude, meter above ocean Planting_date: Date when seedlings where planted for each site Inventory_2019: Date when survival and growth inventory was performed for each site Soiltype_till: All sites are located on till. Coarse, sandy or silty defines dominant particle fraction based on personal assessment Moistclass: dry (10 sites) or mesic (1 site) Site_Index: T16-T24, “T” stands for “Tall” = Swedish for “Pine”. Site index represents the productivity of the sites and is the estimated height of dominant trees at 100 years based on the productivity of the former stand, unit meter. Growingseason_days_SMHI: Length of growing season is the normal value based on 1961-1990 data, where the start of growing season is defined by the first day of the year when the diurnal mean temperature has been above 5 °C for four consecutive days and end is the last day of the last four days period when the diurnal mean temperature has been above 5 °C (SMHI, 2020). Precipitation_30_first_days_mm_SMHI: The amount of precipitation from date of planting and the following 30 days, unit mm. Data is retrieved from the closest available SMHI weather stations as listed in access/sharing information, which means some clustered sites share the same data. SMHI_station: Name and coordinates for the weather station from where precipitation data was retrieved. Potsize_cm3: The size of the pot that seedlings were grown in, defining the substrate size in which all roots are confined at planting, unit cubic cm (cm3). Treatment: Fertilized = one dose of granular arginine-phosphate (arGrow® Granulat, Arevo AB, Umeå, Sweden) was added in the bottom of the planting hole together with each plant at the time of planting, b) untreated: no nutrients added. One dose arGrow® Granulat contains 40 mg N and 22 mg P, the active substance is L-arginine phosphate (C6H17N4O6P). Untreated: no fertilizer added. Position: Tilt= turned over humus tilt with mineral soil cover on top of intact humus. Mineral= bared mineral soil adjacent to the turned over tilt where topsoil was removed. Non_prepared= intact humus where no topsoil or vegetation was removed. Positions “Tilt” and “Mineral” both resulted from mechanical soil preparation, performed in 2017 either by mounding or disc-trenching. Alive2019: Number of seedlings alive at the time of inventory for the specified treatment- and position combination for each site. Dead2019: Number of seedlings dead or missing at the time of inventory for the specified treatment- and position combination for each site. Survival2019: Percentage of seedlings alive at the time of inventory for the specified treatment- and position combination for each site. Mean_Leader_2019_cm: Mean leader shoot length of seedlings for the specified treatment- and position combination for each site. Number_measured: Number of seedlings that were measured for the specified treatment- and position combination for each site. All date variables use the YYYYMMDD date format. 4. Missing data codes: None.