Dataset of confocal microscopy from plant samples - High-throughput characterization of cortical microtubule arrays response to anisotropic tensile stress

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dc.contributor.authorElsa Demes
dc.contributor.authorStéphane Verger
dc.date.accessioned2025-01-20T09:54:08Z
dc.date.available2025-01-20T09:54:08Z
dc.date.issued2023-05-10T10:49:17.488981Z
dc.descriptionThe data set contains Tiff Z-stacks from light-grown hypocotyls and cotyledons of cortical microtubule (CMT) reporter lines either with few or no ablated cells from a time series experiment. This data set was analyzed with a new semi-automated image analysis workflow we have developed to quantify CMTs reorganization in individual cells following an ablation (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). The dataset in the zip file was analyzed using the scripts on GitHub (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). A step by step describes and explains all the scripts of the image analysis procedure. The intermediate data generated by the analysis method can be found on zenodo (https://doi.org/10.5281/zenodo.7436075). The documentation file Example_2D_Image.tif gives a visual representation from a typical z-stack.en_EN
dc.descriptionDatamängden innehåller Tiff Z-stackar från transgena ljusodlade hypokotyler och hjärtblad från Arabidopsis thaliana uttryckande kortikala mikrotubuli (CMT)-reporterlinjer antingen med få eller inga dödade celler från ett tidsserieexperiment. Denna datauppsättning analyserades med ett nytt halvautomatiserat arbetsflöde för bildanalys som vi har utvecklat för att kvantifiera CMT-omorganisation i enskilda celler efter en ablation. (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). Datamängden i zip-filen analyserades med hjälp av skripten på GitHub (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). Ett steg för steg beskriver och förklarar alla skript för bildanalysproceduren. De mellanliggande data som genereras av analysmetoden kan hittas på zenodo (https://doi.org/10.5281/zenodo.7436075). Dokumentationsfilen Example_2D_Image.tif ger en visuell representation från en typisk z-stack.sv_SE
dc.description.sponsorshipSwedish Research Councilen_EN
dc.description.sponsorshipVetenskapsrådetsv_SE
dc.description.sponsorshipBio4Energyen_EN
dc.description.sponsorshipBio4Energysv_SE
dc.description.sponsorshipKnut and Alice Wallenberg Foundationen_EN
dc.description.sponsorshipKnut och Alice Wallenbergs Stiftelsesv_SE
dc.description.sponsorshipKnut and Alice Wallenberg Foundationen_EN
dc.description.sponsorshipKnut och Alice Wallenbergs Stiftelsesv_SE
dc.description.sponsorshipVINNOVAen_EN
dc.description.sponsorshipVINNOVAsv_SE
dc.identifier.govdocSLU.genfys.2023.4.4.IÄ-2
dc.identifier.urihttps://doi.org/10.5878/17te-jg54
dc.identifier.urihttps://doi.org/10.5878/8sgv-nn24
dc.identifier.urihttps://hdl.handle.net/20.500.12703/5491
dc.languageotheren_EN
dc.publisherSwedish National Data Serviceen_EN
dc.publisherSvensk Nationell Datatjänstsv_SE
dc.subjectCell Biologyen_EN
dc.subjectCellbiologisv_SE
dc.subjectDevelopmental Biologyen_EN
dc.subjectUtvecklingsbiologisv_SE
dc.subjectPlant Biotechnologyen_EN
dc.subjectVäxtbioteknologisv_SE
dc.subjectBiological Sciencesen_EN
dc.subjectBiologisv_SE
dc.subjectAgricultural Biotechnologyen_EN
dc.subjectBioteknologi med applikationer på växter och djursv_SE
dc.subjectNatural Sciencesen_EN
dc.subjectNaturvetenskapsv_SE
dc.subjectAgricultural and Veterinary sciencesen_EN
dc.subjectLantbruksvetenskap och veterinärmedicinsv_SE
dc.titleDataset of confocal microscopy from plant samples - High-throughput characterization of cortical microtubule arrays response to anisotropic tensile stressen_EN
dc.titleDatauppsättning från konfokalmikroskopi av växtprover – ”High throughput”-karakterisering av kortikala mikrotubuli-nätverks svar på mekanisk stress.sv_SE
dc.typeDatasetsv_SE
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Dataset of confocal microscopy from plant samples - High-throughput characterization of cortical microtubule arrays response to anisotropic tensile stress